FastQCFastQC Report
Fri 10 Feb 2017
SRR3559585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817097
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454002.4984907244907673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227361.251226544317667No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194831.072204730952723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176200.9696785587120554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.580651445685068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89280.4913331539262902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60950.3354251314046526No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44250.243520296384838No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.21848035630458915No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35440.19503636844923525No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26040.1433055032285013No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.13312442869037813No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23410.12883186753376402No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23190.1276211451562575No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22950.12630035710806853No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT21210.11672464375869862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6000.075.093511
TAACGCA6050.055.104234
TACAACG4250.054.629592
TATCACG2900.053.3737372
GTATAAC6200.050.385331
ACAACGC4900.048.597753
TAGTACT9450.048.475725
ATAGTAC9800.045.5603944
GTACAAC6000.043.0536161
GTATCAA243400.041.144091
AGATTAC11600.040.543512
GCATAGT11100.040.2244872
TCACGCA4150.037.2973144
ACGCATA1652.1464075E-1036.408981
GGTTTCA10450.035.642471
CATAGTA13100.034.9922373
TATCAAC295450.033.791022
ATAACGC10450.033.6115043
AACGCAG318800.033.5534136
ATCAACG297100.033.5031663
Produced by FastQC (version 0.11.5)