FastQCFastQC Report
Fri 10 Feb 2017
SRR3559584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173486
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321942.74344985794462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192021.6363211832096844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168001.4316319069848298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135141.1516115232733923No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78300.6672427280768581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74220.6324745246215123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62380.5315785616530576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58720.5003894379651739No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35810.3051591582686117No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35470.30226180798066615No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32610.2778899790879482No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30170.25709722996269235No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.2541146634898073No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25510.21738648778085123No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.2168751906712138No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT25350.21602302882181806No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21510.18330001380502198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT20670.17614185427009782No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20590.17546012479058123No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC17730.1510882958978633No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17020.1450379467671536No Hit
CTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT16220.13822065197198774RNA PCR Primer, Index 31 (95% over 22bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15510.13217030284127804No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT15300.130380762957547No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.12646081845032664No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.12441563001177687No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC14360.12237044157322713No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC14110.1202400369497378No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.11896179417564419No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13650.11632009244251743No Hit
GCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT13060.11129233753108261RNA PCR Primer, Index 31 (95% over 24bp)
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.10805412250337883No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.10711674446904351No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.10396374562627932No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12080.10294115140700444No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA11940.1017481248178504No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC11840.10089596296845467No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.10012901730399851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3000.075.406582
CGCATAG5400.074.53371
GTATAAC4350.070.429171
GTACAAC3750.065.678641
TAACGCA4150.063.1177444
ACAACGC4100.056.6275333
ATAACGC5850.054.952163
ATAGTAC7850.051.568694
TATCACG2700.050.7120252
TCACGCA3000.049.6095924
GTGTTAT5100.049.4710041
GAGTTCT8900.048.5975231
GCATAGT8250.048.34682
AGTTCTT9000.048.2866672
TAGTACT8450.047.8784335
GGTTTCA5650.046.7816851
ACGCATA2350.046.0125471
AGTGTAC6550.043.626143
ATCTACG2750.043.2956432
GTATCAC3850.042.128371
Produced by FastQC (version 0.11.5)