FastQCFastQC Report
Fri 10 Feb 2017
SRR3559581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13542642
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3537242.611927569229106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790261.321942941414238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1252720.9250189143300104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT664990.49103417191416565No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332640.24562415516854097No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318540.23521259736467964No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307860.22732639613452088No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT277730.20507815240187252No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207950.15355201739808227No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT159950.11810841636366079No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT148290.1094985749457159No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC146000.10780761981303207No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA137440.10148684429522688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA358000.082.8530961
GTATCAA1079300.079.735321
TATCAAC1471850.058.516062
ATCAACG1520650.056.489423
TCAACGC1561500.055.198434
CAACGCA1572200.054.8038255
AACGCAG1618900.053.4662486
ACGCAGA1820950.047.478177
CGCAGAG1845200.046.869988
GCAGAGT1924900.044.7564249
GGACCGT48500.035.8185236
AGAGTAC1876800.034.72174510-11
GACCGTT37100.034.637497
GTACTGG128250.032.8857961
ACGGTAC42550.031.9003473
TACTGGT119850.031.890192
GGTTCAC120400.030.8830436
AGTACTT1325200.030.74969712-13
CAGAGTA1904600.030.42750510-11
AGTACAT814900.029.95804612-13
Produced by FastQC (version 0.11.5)