FastQCFastQC Report
Fri 10 Feb 2017
SRR3559580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538180
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396452.5773966635894365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205481.3358644631967649No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192851.2537544370619824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167261.08738899218557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA106600.6930268239087753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86660.5633931009374715No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77330.5027370008711595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52600.3419625791519848No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT34840.22650144976530703No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.2111586420314918No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT30420.19776619121299197No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.1964009413722711No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29820.19386547738236098No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25870.16818577799737353No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24330.15817394583208727No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.14055572169707056No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21360.1388654123704638No Hit
CTGCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT20340.13223419885839108RNA PCR Primer, Index 33 (95% over 22bp)
GCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT19750.12839849692493727RNA PCR Primer, Index 33 (95% over 24bp)
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.10739965413670702No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15640.10167860718511487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5950.075.747281
TAACGCA4950.056.5417254
GTATAAC5400.054.528691
ATAGTAC9050.053.2982374
TCACGCA3250.053.136264
TATCACG3150.052.932682
TAGTACT9650.051.1685875
TACAACG4200.051.0422252
GCATAGT9500.050.7735822
ACAACGC4600.047.898323
GTACAAC4750.046.8091621
ATCACGC3800.043.8784073
AGATTAC9950.043.0909272
ATAACGC7100.041.93613
GTATCAC5400.040.0618931
GTATCAA245400.038.935461
CATAGTA12550.038.908683
GACCGTT2050.037.727357
GATTACT12250.035.0003853
GGTTTCA8100.033.3849141
Produced by FastQC (version 0.11.5)