FastQCFastQC Report
Fri 10 Feb 2017
SRR3559578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426797
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416631.716789661434393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213210.8785654506742839No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202540.834598031891419No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163040.6718320485809073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100650.4147442081064052No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89470.36867525384282246No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72900.3003959540085141No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65770.2710156638565154No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41920.17273797519940895No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38670.15934583733208835No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38410.1582744663027027No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37700.1553487992609188No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36110.14879695335044504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34510.142203900861918No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29540.12172423156943082No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG28020.1154608317053301No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26860.110680868651148No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT26750.11022759629256175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7050.069.896241
TATCACG3250.060.453322
TAACGCA7100.058.6975984
GTATAAC8750.052.882471
TACAACG6400.052.0951922
AGATTAC15450.048.5547452
ACAACGC7800.045.034673
GATTACT18150.041.659753
GCATAGT12050.041.009142
TAGTACT12000.040.6528475
ATAGTAC12300.039.6907544
ATAACGC14150.039.5513233
GTACAAC9500.038.586491
GTATCAC7750.036.443931
TCACGCA5800.035.926984
AGTGTAC13850.035.2495573
AAACGCA9800.035.2095345
GTGTTAT8200.035.176811
GTATCAA295550.033.833851
ATTACTT22800.033.4237944