FastQCFastQC Report
Fri 10 Feb 2017
SRR3559577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667181
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1045771.3639563224084577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT562290.7333725393987699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423050.5517673314351128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311920.4068248812699218No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244810.31929597070944327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166660.21736802613633355No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA141850.18500932741773018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT108040.14091228575404702No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91280.11905288266965396No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86820.11323588161020327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84470.1101708698412102No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78160.10194098717638204No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77400.10094974932768641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG14200.054.918752
CGCATAG20050.054.8298761
TAACGCA18500.049.8766944
TATCACG11550.047.4180342
GTATAAC21550.043.7656061
ACAACGC17850.043.0218623
GTATCAA652550.041.3621561
AGATTAC35250.036.140352
ATAGTAC30600.034.6287084
TAGTACT32850.033.5044175
TCAACGC825500.033.4104964
ATCAACG804550.033.3925823
AACGCAG866750.033.2769136
CAACGCA834500.033.2290955
GTACAAC25700.033.192181
TATCAAC822400.032.725712
GATTACT41850.030.725293
CATGGGT105200.030.5007654
ACGCAGA951400.030.272357
CATGGGG198100.029.9304054
Produced by FastQC (version 0.11.5)