FastQCFastQC Report
Fri 10 Feb 2017
SRR3559575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175387
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2206323.57276394175782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061891.7195521511445357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT779521.2623014557630154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421680.6828397961131829No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213900.34637505309383854No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199740.32344531605873444No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176480.2857796604488107No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126050.20411676223692538No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT105700.17116336190752093No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC94780.1534802596177373No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA75370.12204903109716038No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC69430.11243020073073962No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68420.11079467570210579No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67170.10877051106270748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA797450.063.8572461
TATCAAC1021750.049.864082
GGTATCA371250.048.273581
ATCAACG1053450.048.222313
TCAACGC1075250.047.4051934
CAACGCA1081650.047.120155
AACGCAG1106800.046.2544446
ACGCAGA1223950.041.7789427
CGCAGAG1230100.041.593918
GCAGAGT1265500.040.322279
GTACTGG51850.034.877111
GGTTCAC45750.033.290526
TACTGGT47950.032.901632
CATGGGT93950.031.1132144
AGTACTT806400.030.95659312-13
GACCGTT11600.030.7729037
AGAGTAC1251050.030.55652610-11
GGACCGT15800.030.4999876
GAGTACT773200.029.03877312-13
ACGGTAC16700.028.8754463
Produced by FastQC (version 0.11.5)