FastQCFastQC Report
Fri 10 Feb 2017
SRR3559574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503960
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT538843.5828080534056754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268511.7853533338652625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204011.35648554482832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202731.3479746801776642No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111650.7423734673794515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94490.6282746881565998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81580.5424346392191282No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43720.29069922072395543No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40730.2708183728290646No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.262839437219075No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29990.19940689911965742No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24980.16609484294795074No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22270.14807574669539084No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT21030.13983084656506822No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20810.13836804170323677No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.13757014814223784No Hit
CTGCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT20500.13630681667065614RNA PCR Primer, Index 33 (95% over 22bp)
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.13378015372749275No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.13025612383308066No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.12600069150775287No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18610.12373999308492246No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18040.1199499986701774No Hit
GCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT17380.1155615840846831RNA PCR Primer, Index 33 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.1081810686454427No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.10459054762094737No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15550.10339370727944891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4250.077.045962
CGCATAG6650.075.897161
GTATAAC5600.066.522961
TATCACG3050.064.4154742
TAACGCA6100.064.4154744
GTATCAC5200.061.2407381
ACAACGC5450.061.1741073
AGATTAC12400.059.5355152
GTACAAC6300.056.2703171
GCATAGT9500.054.5222
ATAGTAC9550.054.2365464
TAGTACT9700.053.3587765
ATAACGC7500.052.3912543
TCACGCA3800.051.7018934
GATTACT15100.049.284373
GGTTTCA9400.049.2187731
GTATCAA249550.047.7455641
AAACGCA6400.045.5485425
AGTGTAC8900.044.818873
ACGCATA2350.043.465931
Produced by FastQC (version 0.11.5)