FastQCFastQC Report
Fri 10 Feb 2017
SRR3559572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194561
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258002.159789244751838No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147431.2341772416812538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123691.0354431460595148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107850.9028421319631228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107560.9004144618818127No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.48285520789645736No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56370.47188883614984917No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31010.259593273177343No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT21260.1779733307884654No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.17412254376293887No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20250.16951834188459192No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19240.16106335298071844No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17650.14775302391422457No Hit
CTGCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT17160.14365109860442457RNA PCR Primer, Index 31 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.14273025822875515No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14110.11811870636995515No Hit
GCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT13730.11493762143582455RNA PCR Primer, Index 31 (95% over 24bp)
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.1095800047046572No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12160.10179471789217964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3300.067.345091
TACAACG2400.057.0627062
TAACGCA3150.056.708284
TATCACG2050.052.2822652
ACGCATA909.02819E-846.716871
GTATAAC4850.042.1071051
ATAGTAC5100.042.030844
AAACGCA5100.041.995665
ATAACGC4750.041.36723
AGATTAC8200.040.6639822
ATCACGC2650.040.444773
GATTACT8350.039.9334953
GTATCAA159250.039.9047361
ACAACGC3500.039.128713
GCATAGT5900.038.3501782
TCACGCA3000.037.7110064
TAGTACT5850.036.61165
GTACAAC4700.035.7831341
GTGTTAT4300.034.921251
CATGGGT16550.034.1791574
Produced by FastQC (version 0.11.5)