FastQCFastQC Report
Fri 10 Feb 2017
SRR3559569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769234
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT639112.307894529678604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316861.1442153317487795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271080.9788988579513325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110720.3998217557635072No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46370.16744702686735755No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.1559637069312308No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.1500776026872413No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31450.11356931194691385No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28060.10132765956217496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267400.062.9129681
GGTATCA126200.054.3106731
TATCAAC349550.048.1260642
ATCAACG357650.047.0194663
TCAACGC361850.046.6875764
CAACGCA365800.046.272265
AACGCAG377100.045.2809336
ACGCAGA419250.040.7001577
CGCAGAG426500.040.034768
GCAGAGT440850.038.596649
ACGGTAC6250.030.4780713
AGAGTAC430950.030.30964710-11
AGTACTT268450.029.33127612-13
GAGTACT257550.027.8237512-13
CAGAGTA439250.027.15669810-11
ATAGACG3800.026.615818
GGACCGT7450.026.3540696
TATCACG2957.2759576E-1226.2324492
GTATTAG7650.025.8899351
TACTTTT305100.025.86613714-15
Produced by FastQC (version 0.11.5)