FastQCFastQC Report
Fri 10 Feb 2017
SRR3559567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914741
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150921.6498659183309812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70990.7760666680513938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65890.7203131815453773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63680.6961533373927702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34840.38087283722933596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29210.31932536094916486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.31549914128698725No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.2962587224143227No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT20900.22847997411289098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18800.20552265613982537No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16430.17961368299879418No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15410.1684629856975909No Hit
CTGCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT14960.16354356041764828TruSeq Adapter, Index 10 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14670.1603732641261297No Hit
GCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT13190.14419382098320727TruSeq Adapter, Index 10 (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.12396951705455424No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11280.12331359368389522No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.11150697301203293No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.11008580570893838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG1600.066.92875
CGCATAG3300.063.6861041
TAACGCA2100.059.547524
ACGCATA1450.057.9763151
TACAACG2250.050.2845732
GTATAAC2950.046.8162231
TATCACG2050.046.4761122
CTACGCA2250.044.991464
TAGTACT4950.044.4689065
ATCTACG2150.041.544782
ACAACGC2750.041.1419223
ATAGTAC5100.040.8659444
TCTACGC2700.039.6983453
GCATAGT5250.039.6983452
GGTTTCA5600.037.529311
TCACGCA2400.037.2171974
ATCACGC2400.037.2171973
AGATTAC5450.037.148912
GTGTTAT3500.036.0281371
TATCTAC2550.035.3217051
Produced by FastQC (version 0.11.5)