FastQCFastQC Report
Fri 10 Feb 2017
SRR3559564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670759
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262021.5682692716304385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140830.8429103180051701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119380.7145255539548193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97900.5859612307939087No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77290.462604121839236No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53000.31722109532254505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.3057293122467094No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46720.27963338817866606No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.24994628189942414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38030.22762109915313938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37870.22666345056348644No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37520.22456859427362053No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31150.18644220979806184No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT31060.18590353246638205No Hit
CTGCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT26350.15771275210847285RNA PCR Primer, Index 39 (95% over 23bp)
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.14903406176474285No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24150.1445450840007446No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22680.13574668758330796No Hit
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT22670.13568683454645464RNA PCR Primer, Index 39 (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.12162137088592669No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20270.12132210570166015No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19970.11952651459606083No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19940.11934695548550091No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC19370.11593533238486221No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19340.11575577327430228No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.10617928737777262No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCTCTC17220.10306692946140047No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA17140.102588105166574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5700.072.671441
TAACGCA5300.065.1416854
GTATAAC6100.056.0963441
ATAGTAC8050.053.9801184
TATCACG3750.052.3828962
TACAACG3800.051.693652
TAGTACT8650.050.8965645
GGTTTCA8700.049.6824231
TCACGCA4150.048.7683034
GCATAGT8700.048.5787052
TCAAACG2550.044.3527223
AGATTAC10650.043.5965232
CATAGTA10350.041.4094053
ATCACGC4800.040.9241373
ACAACGC4900.040.0889553
GTACAAC5500.038.2027741
ATAACGC10250.037.7482073
TACTGGT10150.036.9471862
GAGTTCT12250.035.7747271
TCTAACG1001.059297E-535.7156142
Produced by FastQC (version 0.11.5)