FastQCFastQC Report
Fri 10 Feb 2017
SRR3559563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9452686
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1744561.8455706663693263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT878800.9296828435854104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT627040.6633458468841555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334280.35363493508617555No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184400.19507682789844072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170610.18048838182078616No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143360.15166059678698732No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100880.10672098914530748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA577900.075.23881
GGTATCA212700.072.235221
TATCAAC798350.054.4837042
ATCAACG815550.053.225163
TCAACGC822250.052.9721684
CAACGCA833650.052.231655
AACGCAG862350.050.8390776
ACGCAGA964850.045.358317
CGCAGAG983500.044.5340168
GCAGAGT1031800.042.351749
AGAGTAC1002100.033.54974710-11
AGTACTT635350.030.5545312-13
CAGAGTA1018800.029.09892510-11
GAGTACT604500.028.31982412-13
AGTACAT514450.027.65081612-13
TACTTTT711000.027.30736414-15
GTACATG639500.025.3969821
GTACTTT679050.025.22790114-15
TACATGG653750.024.8127962
ACATGGG646400.024.4963363
Produced by FastQC (version 0.11.5)