FastQCFastQC Report
Fri 10 Feb 2017
SRR3559561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096869
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262862.396457553272086No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170341.5529657598127034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128761.1738867631412684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124641.1363253041156236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104310.9509795609138375No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.5600486475595536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.5060768423576562No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51040.46532448268662896No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32380.29520389399281044No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26390.24059390866183655No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25560.23302691570278675No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24820.22628044005254958No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT24140.2200809759415208No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.1986563573225244No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20620.1879896323079602No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.17741407588326408No Hit
CTGCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT16770.15288972520875327TruSeq Adapter, Index 10 (95% over 21bp)
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.15088401623165573No Hit
GCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT15760.14368169763207822TruSeq Adapter, Index 10 (95% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15000.1367528848021049No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13880.12654200273688107No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.11086100527957303No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12120.11049633092010075No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11910.10858179053287129No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.10739659886458638No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.1021999892421064No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT11080.1010147975738215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4400.075.0814061
GTATAAC3300.074.6263661
TACAACG2850.070.9901052
TATCACG2150.058.122532
GGTTTCA5400.057.8404961
TAACGCA4350.054.7185364
GCATAGT6200.054.7075042
GTACAAC3850.054.604661
ACAACGC3750.053.9524773
TAGTACT6150.053.2025535
ATAGTAC6450.051.664484
CATAGTA6550.049.9672133
ATCACGC2750.047.6051253
GTATCAC3850.045.2438621
TACGCAG3250.043.931175
GAGTTCT6450.043.7683871
TCACGCA3400.043.7547074
TAAGACG705.4483473E-542.5045784
ATAACGC5700.041.7588843
TTCAACG8400.040.3793454
Produced by FastQC (version 0.11.5)