FastQCFastQC Report
Fri 10 Feb 2017
SRR3559557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10054476
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1824951.8150622667954055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT921700.9167061515687143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT692460.6887081932464705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352940.3510277412766215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200940.19985129011198594No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191650.19061162411646315No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145170.1443834566813825No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.10699712247560192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA630200.072.689981
GGTATCA241050.072.458021
TATCAAC852750.053.756152
ATCAACG875550.052.179513
TCAACGC889750.051.4805684
CAACGCA896300.051.1428075
AACGCAG928050.049.6246536
ACGCAGA1035950.044.3985377
CGCAGAG1056250.043.5337648
GCAGAGT1104100.041.5878039
AGAGTAC1071500.032.7061610-11
CAGAGTA1093600.029.00689910-11
AGTACTT695000.028.80919612-13
GAGTACT643750.028.4226712-13
AGTACAT553900.027.14190112-13
TACTTTT770050.026.0475814-15
GTACATG691600.025.69741
CATGGGT167150.025.216574
TACATGG704500.025.0047842
ACATGGG687000.024.8184933
Produced by FastQC (version 0.11.5)