FastQCFastQC Report
Fri 10 Feb 2017
SRR3559552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879788
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428542.279725160496822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269921.4359066022338691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228601.2160945808782693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183930.978461400966492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121290.6452323347100843No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101760.5413376402019802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.5185691152406549No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64750.3444537362723881No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40410.21497104992690666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38710.20592747692824934No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37190.1978414587176852No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34710.184648481637291No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34290.1824141871317404No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT34150.18166942229655686No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31870.16954039498071058No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31510.16762528540452434No Hit
CTGCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT28150.14975092936011933RNA PCR Primer, Index 39 (95% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25910.13783469199718268No Hit
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT24930.13262133815089786RNA PCR Primer, Index 39 (96% over 25bp)
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.11863039874709276No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22210.1181516213530462No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.11804522637659141No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.11336384741258057No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20500.1090548508661615No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.10846967849566015No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19990.10634177896656431No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.10612898901365474No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19830.10549061915492597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3900.074.794542
TACAACG4800.073.172732
CGCATAG8350.071.9565051
GTATAAC6550.066.9634861
TAACGCA6500.065.941324
ACAACGC5350.065.65033
TCACGCA4950.058.9290434
GTACAAC6750.056.07811
ATAGTAC11900.052.5267834
TAGTACT12400.050.8563655
ATAACGC10200.050.192263
GCATAGT12400.049.9286882
GTATCAC7350.049.04791
ATCACGC5950.049.0249983
AGATTAC14100.048.5531272
ACGCATA2250.048.0669441
GGTTTCA10350.047.022011
CATAGTA14350.043.5587963
GATTACT16900.040.508823
GAGTTCT14900.040.3246151
Produced by FastQC (version 0.11.5)