FastQCFastQC Report
Fri 10 Feb 2017
SRR3559551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11132107
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1813891.6294219953149929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT947490.8511326741649178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT697030.6261438198536899No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367750.3303507592947139No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT192840.17322866192356937No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171280.1538612591488745No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170780.1534121078785894No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143900.12926573558806073No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC130520.11724644759523062No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT115400.10366411318180825No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA111670.10031344470548118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA682150.065.265151
GGTATCA312700.054.73741
TATCAAC903450.049.208322
ATCAACG917350.048.2680173
TCAACGC938000.047.4531254
CAACGCA942850.047.2167785
AACGCAG976950.045.8561446
ACGCAGA1093100.040.940057
CGCAGAG1109300.040.3741658
GCAGAGT1163950.038.3561829
TACTGGT87950.034.317092
GTACTGG106700.033.546641
GGTTCAC89850.031.4521566
AGAGTAC1120700.030.90182310-11
ACGGTAC31300.030.0504573
AGTACTT773350.027.67923512-13
GAGTACT692600.027.39738812-13
ACTGGTT104350.027.3263363
CAGAGTA1143450.027.05850410-11
GACCGTT25050.026.837727
Produced by FastQC (version 0.11.5)