FastQCFastQC Report
Fri 10 Feb 2017
SRR3559550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511554
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638611.8185965529791084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321350.9151219089895811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245850.7001173839274577No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225390.6418525815066493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140520.40016471340039195No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105670.30092090282535877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93010.26486848842421334No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54280.15457543868042467No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.14460834149211432No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49120.1398810896828014No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35250.10038290739655435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9750.062.8296471
TAACGCA7700.048.6992654
TACAACG7950.048.6652262
TATCACG4250.047.6170542
GTATCAA323750.047.4711341
ACAACGC9200.043.3470463
AGATTAC18000.040.0115552
TAGTACT15650.039.154815
ATAGTAC16100.038.0788574
ATCAACG407400.037.577033
TCAACGC416800.037.4150284
TATCAAC411500.037.332812
AACGCAG435950.037.010736
CAACGCA423150.036.9903765
GTACAAC13250.036.2612881
GCATAGT18050.033.9650732
ACGCAGA477400.033.8969887
GTATAAC12100.033.7514651
CGCAGAG483950.033.5611468
GATTACT22200.032.7099153