FastQCFastQC Report
Fri 10 Feb 2017
SRR3559546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470969
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351352.388561553642531No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200181.3608716431141648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192801.3107006333920022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153121.0409464781378805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79110.5378087505583055No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77050.5238043765708184No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62250.4231904275345028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57570.39137466527166787No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37330.2537782917247066No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36990.2514668901927913No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT34910.23732655140930908No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32230.21910726874597627No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30610.20809412027037957No Hit
CTGCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT29260.1989164965407157RNA PCR Primer, Index 39 (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.1926621159249447No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27370.1860678233191862No Hit
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT26630.18103712586737042RNA PCR Primer, Index 39 (96% over 25bp)
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.17865774193745756No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23590.16037047687612724No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19880.13514900721905085No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC18790.1277389258373222No Hit
GTACATGGGCCATCTCATCCCTGCACATCTCCGAGCCCACGAGACCTCTC18150.12338805236548153No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT17670.12012489726160103No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17500.11896919649564335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.11230692149188733No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16190.11006350235796948No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.10951964317398939No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.10945166077599187No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16090.10938367837799437No Hit
CAGATTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.10319728016022092No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC15180.10319728016022092No Hit
GTGTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.10224552658825578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6750.078.333321
TATCACG2700.068.348512
TACAACG3650.065.237652
GTATAAC4900.061.3114551
ATAGTAC9000.060.1907924
TAGTACT9450.057.2914355
GCATAGT9500.057.0228542
TAACGCA5150.055.4836654
AGATTAC11350.055.0712052
ACAACGC4450.054.8472673
GTATCAC4850.053.271441
GTACAAC5200.049.685861
GTGTTAT5000.049.2698821
CATAGTA11350.047.728383
ATCACGC4000.046.1352463
TCACGCA4300.044.3009154
GATTACT14150.044.1737253
TCTAACG1109.59335E-943.294072
GGTTTCA8800.043.0155561
GAGTTCT10600.041.946291
Produced by FastQC (version 0.11.5)