FastQCFastQC Report
Fri 10 Feb 2017
SRR3559535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738966
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402082.3121786164881892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186811.0742590711951816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143400.8246279685744287No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98880.5686137624312378No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77480.44555212695360347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52910.3042612679028802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43840.2521038364177333No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31030.17843937144256988No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.16797338188325706No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26010.14957164199875098No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21210.12196903217199186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20920.12030137449495851No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20000.115010874278163No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.11483835796674574No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT18820.10822523269575139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5050.061.7882581
TAACGCA4100.047.9511264
GTATCAA198500.047.1885221
TATCACG2100.042.5540352
TACAACG3700.041.8639682
TAGTACT7450.040.722355
AGATTAC9100.039.9353262
ATAGTAC7650.039.7170944
GTATAAC5550.038.922751
TATCAAC243450.038.4935262
ATCAACG244650.038.2560163
TCAACGC250850.038.1417164
CAACGCA253350.037.9436765
AACGCAG260500.037.815656
ACGCAGA285300.034.695297
CGCAGAG288550.034.5725148
ACAACGC4400.033.84983
GCATAGT9350.033.7701532
GATTACT10800.033.6492083
GCAGAGT298350.033.4368979