FastQCFastQC Report
Fri 10 Feb 2017
SRR3559533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4153590
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1062112.5570891686468813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT465071.1196820100202476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319740.7697919149458661No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175250.42192416680510114No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159100.3830421394504513No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103070.24814678386648656No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA91150.21944871785611963No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.19274892322063564No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76800.18490029107350509No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69280.16679547090589106No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC59700.14373108563916998No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53380.12851533251957947No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52090.12540958544295416No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48000.11556268192094069No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44010.1059565339862625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA475800.052.9382971
CGCATAG8300.046.265141
TATCACG7300.043.2362442
TAACGCA10250.042.994024
TATCAAC588750.042.7761542
ATCAACG594750.042.255013
TCAACGC607750.041.909694
CAACGCA615200.041.5226975
AACGCAG633300.041.2565276
TCACGCA8400.038.2837684
ACGCAGA686150.037.999847
CGCAGAG694150.037.6647388
TACAACG7950.036.7048872
GCAGAGT719450.036.282359
GTATAAC13800.033.91311
AGATTAC18200.032.7207762
ATAACGC17000.030.4768813
GTATCAC14100.029.7872831
ATCACGC10450.029.6337933
CATGGGG141700.029.5451134