FastQCFastQC Report
Fri 10 Feb 2017
SRR3559530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588231
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1232482.2054922210624435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585281.0473439626958871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390200.6982531681313818No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253370.45339929576998517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235660.4217076924701216No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161810.2895549593422319No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA160120.2865307464920473No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT111040.19870331058254392No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104140.18635593267350617No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97360.17422329177158208No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76420.1367516840302414No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC75060.1343179979496195No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59380.10625902902009599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG9950.049.076152
CGCATAG12150.048.870411
TAACGCA12000.044.166434
GTATCAA621050.043.0720331
TACAACG14100.040.1221542
ACAACGC15400.036.3488543
TATCAAC751100.035.6220052
GTACAAC19250.035.519041
TCACGCA13950.035.431334
ATCACGC13800.035.384933
ATCAACG757750.035.2546923
TCAACGC774100.035.2178084
AACGCAG801800.034.9903376
CAACGCA780700.034.968285
ACGCAGA867900.032.2711877
GTATCAC18800.032.221861
CGCAGAG879200.031.9443828
GTATAAC18900.031.0993521
GCAGAGT915000.030.6815329
CATGGGG135800.030.0820874