FastQCFastQC Report
Fri 10 Feb 2017
SRR3559529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520030
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT794582.2573103070144285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374731.064564790640989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265230.7534878964099737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155240.4410189685883359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143230.40689994119368306No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.27465675008451634No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83780.2380093351477118No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60700.17244171214449877No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.1713621758905464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54190.15394755158336718No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC42040.11943080030567921No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41480.11783990477353887No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.11147632264497746No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT36070.10247071757911155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9400.059.9952131
TATCACG5650.051.650192
GTATCAA361200.049.2485661
TACAACG8050.048.088562
TAACGCA8850.045.0874674
ACAACGC9000.042.350813
GTATAAC10400.040.3813931
TATCAAC453850.039.0783842
ATCAACG454400.038.9393423
TCAACGC466700.038.7297944
AACGCAG488900.038.341146
CAACGCA474300.038.3172845
TAGTACT15450.038.1191645
ATAGTAC15150.037.7383464
AGATTAC16950.035.838912
ACGCAGA531600.035.2609447
CGCAGAG540000.034.921758
AAACGCA12000.034.2061735
GCATAGT16900.034.182932
ACGCATA3700.034.051341