FastQCFastQC Report
Fri 10 Feb 2017
SRR3559527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4899457
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870581.7768907860605778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410520.8378887701228932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284620.5809215184458196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133630.27274451025899404No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89680.18304069206036505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85630.1747744699055426No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68510.13983182217947826No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67280.13732133989542106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61700.12593232270433233No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC58110.11860498010289712No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51020.1041339887256894No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50200.10246033386965127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA430200.052.6470221
CGCATAG9400.049.1201971
TATCACG7250.047.644542
TATCAAC544400.041.4723632
ATCAACG546500.041.2258223
TCAACGC560100.040.894684
CAACGCA567750.040.5083435
AACGCAG585300.040.401586
TACAACG9500.037.614112
ACGCAGA643050.036.6618737
CGCAGAG651050.036.3393028
GCAGAGT676450.034.790129
CATGGGG155800.033.9826664
ACAACGC11100.033.8018653
CATGGGT84850.032.708244
TAACGCA10600.032.025224
AAACGCA15200.031.3102235
ATCACGC12100.031.0083243
GGTATCA241950.030.1373581
ACATGGG440200.029.9672433