FastQCFastQC Report
Fri 10 Feb 2017
SRR3559525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170843
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405451.867707614046709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185160.8529405396889597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138050.6359280703394948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63840.294079304675649No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47880.2205594785067368No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46100.21235989889641949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.14459820447632554No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.13953104853736542No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29780.13718173078384757No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24210.11152349571111315No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.10682486020407739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4450.057.9242861
GTATCAA202450.049.30021
TATCAAC257450.038.5310552
ATCAACG258100.038.3656963
TCAACGC264500.038.090224
AACGCAG275400.037.9331676
TAACGCA4250.037.8280224
CAACGCA268450.037.7406855
AAACGCA6650.035.7846575
ACGCAGA298500.034.7768027
CGCAGAG303800.034.248428
AGATTAC11400.033.949812
GCAGAGT319050.032.5554739
TACAACG4400.032.477862
GATTACT12000.031.7568593
TATCACG3800.031.3382852
TAGTACT8550.031.3115775
CATGGGG64000.030.8885084
ATAGTAC8400.030.4809174
CATGGGT27200.030.209884