FastQCFastQC Report
Fri 10 Feb 2017
SRR3559505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941329
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT854832.9062712807713793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404101.3738687511665646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286690.9746954522938441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154120.5239808263543453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135450.46050611815271264No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.2963286323971239No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76140.25886257538684043No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67800.23050804585274207No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61780.21004110726817707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52100.17713081399598618No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37600.12783337056140268No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.10638048310814602No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30020.10206270702801352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7100.060.838781
TACAACG6650.056.4174232
GTATCAA356200.051.3029481
TAACGCA6450.048.9348074
GTACAAC9650.043.5188141
GTATAAC8100.042.2178541
AGATTAC13150.042.116432
ACAACGC8950.041.254413
TATCAAC447550.040.796692
TATCACG6150.040.669472
ATCAACG452350.040.2196543
TCAACGC463650.039.9073334
CAACGCA470800.039.3482935
AACGCAG482600.039.175126
ATAGTAC11100.038.6288074
TAGTACT11400.038.0944565
GATTACT15500.036.8842773
ACGCAGA526950.035.7989737
CGCAGAG535600.035.320788
GCAGAGT551600.034.2638939