FastQCFastQC Report
Fri 10 Feb 2017
SRR3559500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5496053
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1549972.820151115718862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT751401.3671629440254671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498750.9074694148691798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284580.5177897665834008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283040.5149877557585416No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179370.32636148159415496No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA166880.3036360821120175No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126940.2309657494205387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125720.22874597461123464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125690.22869138998477634No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG81400.14810628645684457No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC79240.14417619335184723No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC74010.13466027347261753No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA61840.11251711000603525No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT60400.10989704793603701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8750.050.1156771
GTATCAA696700.048.464821
TAACGCA10350.044.879024
TACAACG9850.043.5296752
TATCACG8000.041.6857072
TATCAAC862250.038.938662
ATCAACG875950.038.2344783
TCAACGC895350.037.997984
AACGCAG924000.037.626396
CAACGCA905650.037.6136325
AGATTAC20150.035.4646222
ACGCAGA1005450.034.5664987
CGCAGAG1018200.034.2265178
GCAGAGT1051450.033.2403569
AAACGCA17150.032.954435
CATGGGG167550.031.845844
ACAACGC13800.031.5016213
GTATAAC16650.030.6664491
CATGGGT71150.030.4660134
TCACGCA11750.030.4090254