FastQCFastQC Report
Fri 10 Feb 2017
SRR3559489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221031
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT689593.1048193384063527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314091.4141630621094436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232591.047216360329955No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127050.5720316375593137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123360.5554177316750645No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72020.32426382162158024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63040.28383214822305497No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51860.2334951650832429No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50340.22665149653471744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45200.20350909104825643No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36500.16433809343498582No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC31370.14124071208371247No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31020.13966486735214412No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27370.12323105800864553No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT26860.1209348271140745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6000.060.1318051
TATCACG4500.054.257112
GTATCAA312900.050.2731861
TAACGCA6050.049.2152754
TACAACG5150.047.4091262
GTATAAC7350.042.5422331
AGATTAC11650.040.893042
TATCAAC385700.040.745072
GTACAAC7550.040.618841
AACGCAG414650.040.101896
ATCAACG391350.040.080743
TCAACGC402000.039.9965974
CAACGCA406400.039.715575
ACAACGC6450.039.700333
GGTTTCA12700.039.2987371
TCACGCA6050.038.3879174
GATTACT12950.036.787953
ACGCAGA452100.036.714237
CGCAGAG456350.036.437498
AAACGCA7850.036.377795
Produced by FastQC (version 0.11.5)