FastQCFastQC Report
Fri 10 Feb 2017
SRR3559480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4480998
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT733851.6376932103071684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350350.7818570773742813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237470.5299489087029273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA153840.34331637728916636No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT117470.26215142251793017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116990.2610802325731902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79470.17734888522601439No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71820.1602767954817208No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC63260.1411739081338577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.1276724515386974No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC56950.12709222365196324No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54730.12213797015754078No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA51970.11597862797528585No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50750.11325602019907173No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT47400.1057800070430739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8100.048.218161
GTATCAA480050.042.65751
TATCACG7750.037.6440242
TATCAAC578550.035.329262
ATCAACG582600.034.971253
TCAACGC592650.034.9709474
AACGCAG606850.034.9686786
CAACGCA594700.034.9628375
CATGGGG132500.033.611464
TACAACG9550.032.4191472
ACGCAGA657050.032.287947
CGCAGAG667750.031.8860248
GCAGAGT690600.030.8051649
ACAACGC10900.030.588853
TAACGCA6350.030.0039124
AGATTAC17200.029.0771942
TCACGCA10600.028.084444
AGTACTT379050.027.31620812-13
ATAGTAC14300.027.0631874
GTACTGG32650.026.6849921
Produced by FastQC (version 0.11.5)