FastQCFastQC Report
Fri 10 Feb 2017
SRR3559472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291491
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2501832.69260337226824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1165111.2539537518790043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT763410.821622708346809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419200.4511654803303367No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359880.3873221208522938No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244900.2635744898208479No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA236470.25450167255180034No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT193400.208147432957746No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192190.2068451661848459No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183780.19779387398642478No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG123920.13336933760146782No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC109030.11734392252007778No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC103360.11124156499747995No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT103200.11106936443246837No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA93850.10100639391460423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1073350.051.7835431
TATCAAC1331200.041.6665572
ATCAACG1352300.040.893143
TCAACGC1371800.040.832734
CAACGCA1386700.040.473085
AACGCAG1412750.040.2278986
TATCACG13450.038.0736272
ACGCAGA1537400.036.9546747
CGCAGAG1554700.036.619998
TACAACG16850.036.398662
CGCATAG17500.036.0571
TAACGCA15050.035.6085324
GCAGAGT1615100.035.3057639
AGTACTT988000.029.72405612-13
TCACGCA18350.028.555824
CATGGGG271150.027.9774324
GTACAAC27750.027.7194961
GAGTACT928450.027.34713212-13
AGAGTAC1592700.027.11973410-11
GTACTGG70850.026.7183131
Produced by FastQC (version 0.11.5)