FastQCFastQC Report
Fri 10 Feb 2017
SRR3559468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157705
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1433582.3281076310086304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682921.1090495566124066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430900.6997736981554004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263140.42733453453843595No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262890.42692853912293627No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA181600.2949150698190316No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166630.2706040643389055No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT135080.21936744290283477No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114460.18588094103241387No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113590.18446807698647466No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG82350.13373488986562362No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC74330.12071055693639109No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT66030.10723150914179877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65980.10715031005869881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13450.052.2783161
GTATCAA664800.048.553681
TAACGCA12500.040.0118374
TATCAAC816800.039.392982
ATCAACG828250.038.6830563
TCAACGC846200.038.460584
CAACGCA855400.038.1330265
AACGCAG874150.037.9820486
TACAACG12050.037.058982
TATCACG9600.036.5931662
ACGCAGA955350.034.778677
CGCAGAG966700.034.4872638
GCAGAGT1007050.033.1408849
CATGGGG185600.032.657964
ACAACGC14400.031.4246393
ATAGTAC23150.030.8638044
TAGTACT23800.030.2454975
GTACTGG44200.029.6409571
GTATAAC18200.028.3978231
AGTACTT613750.028.28872912-13
Produced by FastQC (version 0.11.5)