FastQCFastQC Report
Fri 10 Feb 2017
SRR3559467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084573
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1149682.26111415845539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT574221.129337704464072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367720.7232072388379516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197720.3888625455864239No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170660.33564273735473954No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114250.22469930119992376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103180.20292756146878016No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92150.18123449107722517No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79700.15674865913027505No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT75560.1486063824828555No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53830.10586926375135139No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC53750.1057119250721742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8700.053.9088481
GTATCAA503100.052.766841
TATCAAC638700.041.429472
TATCACG9100.040.5678982
ATCAACG650350.040.5408363
TCAACGC663200.040.3837974
CAACGCA670800.039.9807855
AACGCAG687200.039.7538576
ACGCAGA751000.036.3528637
CGCAGAG761800.035.930858
TAACGCA10050.035.5481834
GCAGAGT793800.034.5198639
TACAACG8950.033.929652
CATGGGG147500.030.5587864
CATGGGT71750.030.12434
GGTATCA281350.030.1126371
TCACGCA13650.030.0987664
ATCACGC13650.029.6625523
AGTACTT506250.028.80838212-13
AGAGTAC780100.027.58001110-11