FastQCFastQC Report
Fri 10 Feb 2017
SRR3559466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514013
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1642272.9783571420669483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT778821.4124377291094525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521400.9455908065505105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271740.4928171188569922No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227220.41207737450020515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA177750.32236050223312857No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150610.2731404514280253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT137380.24914703683143297No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123260.22353955277218243No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115520.20950258913063863No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG91690.1662854258776684No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC82150.14898405208692833No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC80630.14622743907205152No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA67280.1220163971321794No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT66410.12043859889340122No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59200.1073628226846763No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG56110.10175891859522275No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55460.10058010381912412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10300.060.1270181
GTATCAA767800.050.729721
TATCAAC952250.040.8538132
ATCAACG966700.040.138423
TCAACGC984400.039.8462074
CAACGCA995200.039.5081375
AACGCAG1010100.039.4730956
TACAACG11150.038.452852
ACGCAGA1097150.036.308697
CGCAGAG1107800.036.0234228
TAACGCA9800.035.850634
GCAGAGT1139000.035.067999
AGATTAC20550.035.062632
ATAGTAC18500.034.119694
CATGGGT89350.033.8563424
TATCACG7750.033.8081862
TAGTACT19450.033.0343135
CATGGGG196500.032.0925564
GGTTTCA29200.030.8871671
GTACTGG41150.030.3922731
Produced by FastQC (version 0.11.5)