FastQCFastQC Report
Fri 10 Feb 2017
SRR3559463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317651
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524732.264059601726058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236861.0219830336836737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165350.7134378730878809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.3912582179111523No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83350.3596313681395516No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54390.23467726590414173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.22971534540791516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.18980424576435365No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.16063678267349138No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37210.1605504884039918No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG27290.11774853073219393No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24520.10579677440650037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA234050.051.674111
TATCACG4000.050.6067052
CGCATAG4700.044.763481
TACAACG4000.044.6529732
TATCAAC292650.040.810462
ATCAACG294100.040.4877933
TCAACGC301850.040.158344
AACGCAG312650.039.8238956
CAACGCA305600.039.7303665
CATGGGG73550.036.7504234
ACGCAGA341600.036.3966457
CATGGGT29700.036.083214
CGCAGAG348650.035.8313038
GCAGAGT361700.034.587869
TAACGCA4350.032.8481644
GTATCAC8650.031.9665221
ACATGGG214050.031.7365383
TCACGCA6200.031.6892094
ATGGGGG45750.031.0770455
TACATGG242800.030.1609882