FastQCFastQC Report
Fri 10 Feb 2017
SRR3559461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7603340
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1896792.4946799695923105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT896951.1796789305752473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT607020.798359668251058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342310.4502100392722146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329180.4329413126336583No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA288420.3793332930001815No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT231200.30407689252354886No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216570.28483534867571353No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153900.2024110456720336No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153240.20154300609995082No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC133790.17596214295296542No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC131580.1730555255979609No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG121180.1593773262802926No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT118100.15532647494390625No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA104990.13808405253480707No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC99800.13125810499070145No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA97890.12874605107755277No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG95410.12548432662487802No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT88890.11690914782187828No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT84190.1107276538994705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15650.048.527551
TACAACG15200.043.2526362
TAACGCA13050.042.5927734
GTATCAA1017600.042.5285381
TATCACG12350.042.1032642
TATCAAC1225500.035.3531652
ATCAACG1245850.034.7085343
TCAACGC1269000.034.5887224
AACGCAG1304400.034.4867866
CAACGCA1280450.034.3854455
TCACGCA15150.034.3218044
GTACTGG64300.033.201391
GACCGTT13050.032.9569637
ACGCAGA1409700.031.8863667
CGCAGAG1424000.031.6164978
TACTGGT54250.031.5086462
ACAACGC20250.030.990443
GCAGAGT1465450.030.670679
GTATAAC20300.030.6362971
GTACAAC26500.030.012681
Produced by FastQC (version 0.11.5)