FastQCFastQC Report
Fri 10 Feb 2017
SRR3559460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8127588
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2576873.170522423134637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200721.4773386643122166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800170.98451102590338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432410.5320274600533392No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324030.3986791653317072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220770.27163040252532483No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA212130.2609999424183411No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186360.22929311869646934No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185760.22855489230015102No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT176960.21772757182081573No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC157380.19363678375429463No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT151620.18654981034963877No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC132610.1631603373596201No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG102200.12574456283955338No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA93340.11484341972058623No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT91510.11259182921181538No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG88820.1092821142016549No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA88130.10843315384588884No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA81540.10032496725965934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1020900.054.6526681
TATCAAC1275150.043.6023642
ATCAACG1304800.042.542873
TCAACGC1331150.042.140594
CAACGCA1348000.041.7219435
AACGCAG1375000.041.4542966
CGCATAG14800.039.9938621
TACAACG12150.039.8306162
TAACGCA12550.039.5132374
ACGCAGA1501250.037.9688387
CGCAGAG1516550.037.6606038
GCAGAGT1568350.036.4072579
GACCGTT19300.035.277477
TACTGGT70800.032.6577262
GGTATCA556100.032.5016251
GTACTGG80250.032.4833871
AGTACTT1025650.029.99333612-13
GGTTCAC74900.029.981126
TCACGCA16850.029.7843174
TATCACG16450.029.782162
Produced by FastQC (version 0.11.5)