FastQCFastQC Report
Fri 10 Feb 2017
SRR3559458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547327
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333672.156428473102324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153240.9903530410831066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118300.7645442753858751No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100980.6526093062423134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70950.4585326824905143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64980.4199500170293674No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49620.3206820536318438No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42940.27751082996677495No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32840.21223697382647624No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32200.20810080868491274No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31410.20299522983829532No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26440.1708753224108414No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25710.16615750904624557No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.161181185360302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24770.1600825164945742No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20600.1331328154940746No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20040.12951367099520658No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16550.1069586454576182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4150.053.2926861
TATCACG3200.050.4246752
TACAACG3850.048.1205182
GTATCAC5000.040.6464771
ATCACGC4050.039.841723
TATAGCG600.001284720439.815855
TCACGCA4100.039.3558464
ACAACGC4700.038.1463283
AGATTAC7100.037.0359652
TAGTACT6350.035.7402085
GTATCAA232800.034.6115381
GATTACT7600.034.599393
ATAGTAC6600.034.4087564
TAACGCA3100.032.773034
GCATAGT7400.030.6888942
GTACAAC6550.030.1152611
TATCAAC269900.029.8480762
TCAACGC277650.029.4884684
AACGCAG285350.029.4485236
ATCAACG273900.029.4339963
Produced by FastQC (version 0.11.5)