FastQCFastQC Report
Fri 10 Feb 2017
SRR3559447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951622
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1155611.6623602376538884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551110.7927790089852411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364630.5245250676748535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204660.29440611126439264No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195550.2813012560234144No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129880.18683409425886505No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125610.1806916429000311No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94520.13596826754964525No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88980.1279989044283478No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86390.12427315524348131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA565550.049.0957451
CGCATAG13900.041.7233851
TATCAAC703950.039.3905032
TCAACGC713700.039.346414
ATCAACG703850.039.30273
CAACGCA722800.038.9729965
TAACGCA12050.038.6832434
AACGCAG743250.038.599876
TATCACG10650.035.351372
ACGCAGA811550.035.307657
CGCAGAG827350.034.7416768
GCAGAGT861200.033.329829
TACAACG13550.029.9905872
AGTACTT510650.028.12287112-13
GAGTACT469350.026.32235112-13
AGAGTAC840100.026.21611210-11
CATGGGG189600.025.8458524
ATAGTAC22950.025.7791064
GGTATCA332700.025.3389681
TAGTACT24200.024.9295335
Produced by FastQC (version 0.11.5)