FastQCFastQC Report
Fri 10 Feb 2017
SRR3559436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11591818
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2431702.097772756611603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1151320.9932178024189131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT749750.6467924185835217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416970.35971061657455283No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348610.3007379860518859No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246550.21269312544417107No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA201920.17419183082412096No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193170.16664340313141562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189090.16312367913298845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166490.14362716874954387No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC116790.10075209945497764No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT116400.10041565524924563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1049900.054.569621
TATCAAC1310950.043.5720522
ATCAACG1327200.042.908133
TCAACGC1350050.042.606374
CAACGCA1362000.042.319415
AACGCAG1389600.041.7923166
ACGCAGA1520450.038.1526687
CGCAGAG1542700.037.668158
GCAGAGT1622300.035.8576779
TATCACG15850.031.6357122
GGTATCA570800.031.0966241
CGCATAG17100.030.077081
TAACGCA15850.029.3760174
AGTACTT1017650.028.85366412-13
CATGGGT148100.028.4159154
CATGGGG326750.028.115814
AGAGTAC1589600.027.55682210-11
GAGTACT943350.027.46432512-13
GTACTGG83950.025.788211
TACAACG21100.025.7446252
Produced by FastQC (version 0.11.5)