FastQCFastQC Report
Fri 10 Feb 2017
SRR3559434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229392
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1799944.255789011753936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT853062.016980218433288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT572201.352913137396581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285780.6756999587647586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196080.4636127367716211No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151780.35886954909831015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124790.2950542300169859No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124180.29361194233119087No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT85300.20168383540707505No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC76910.1818464687122877No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64400.15226774912327823No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT53470.12642479108108212No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52410.12391852067625796No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50540.11949708137718142No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47260.11174182955848028No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT45820.1083370848575871No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG43860.10370284901470471No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA42650.10084191770353752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA562000.071.037861
GGTATCA228650.055.803261
TATCAAC744500.053.6311262
ATCAACG765800.052.1238333
TCAACGC780000.051.3586354
CAACGCA788500.050.8435635
AACGCAG802000.050.1662946
ACGCAGA886300.045.334697
CGCAGAG893000.045.0079238
GACCGTT9400.043.805047
GCAGAGT931200.043.1359529
TACTGGT35300.038.7356642
GGACCGT15650.037.368826
GTACTGG39250.037.3393171
CATGGGT96000.036.5104454
GGTTCAC38450.034.1447566
AGAGTAC927850.033.1768410-11
ACTGGTT40500.033.1724663
TGGTTCA41000.032.457775
AGTACTT654900.032.39350512-13