FastQCFastQC Report
Fri 10 Feb 2017
SRR3559430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9705578
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460522.53516070861519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1161651.1968890466904702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT742170.7646839786357906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436950.45020502642913174No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA416620.42925830898479206No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387230.39897675336801164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT347580.3581239571718449No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267670.27578986022264723No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202750.20890049000688057No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195630.20156450239233564No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG178710.18413122845440014No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC176360.18170994040746466No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA156160.1608971665572107No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC148040.15253084360354427No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG146010.15043926286512765No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT138170.14236143380641522No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC120940.1246087559133521No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC116060.11958071945843926No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG112930.11635576984698902No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT108930.1122344284905031No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT98150.10112741353477352No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC97990.10096255988051407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1359800.040.8482971
CGCATAG17950.039.30951
TATCACG14000.035.8576242
CATGGGT135450.034.988434
TAACGCA16400.034.2542344
TATCAAC1621850.034.2211882
ATCAACG1653450.033.4804233
TCAACGC1678500.033.379534
AACGCAG1714400.033.247386
CAACGCA1689350.033.2418065
ACGCAGA1845600.030.8973187
CGCAGAG1865250.030.6390768
GCAGAGT1913200.029.8197069
TACTGGT68500.028.8780382
CATGGGG264850.028.7700424
GTACTGG84000.028.4036181
AGTACTT1037750.028.13868912-13
GAGTACT956450.026.45274512-13
ATGGGTG85950.025.7149685
ATAGTAC27300.025.6125894
Produced by FastQC (version 0.11.5)