FastQCFastQC Report
Fri 10 Feb 2017
SRR3559429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116988
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT935992.2734824585352205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462681.1238313057992884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303320.7367522081677187No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197380.47942816447363945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173670.4218375181078983No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA142810.34687980630499776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115090.2795490295332413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT106000.25746978130613934No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75730.18394515602182956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.1798402132821373No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC69550.16893418197964144No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG64740.15725088341282512No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56310.13677474891838404No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG48380.1175130945244436No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44470.10801586013852846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42000.10201632844205522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG6300.052.1716882
CGCATAG8650.051.8311041
TACAACG9150.043.7589072
GTATCAA516650.041.57251
ACAACGC10800.036.5201843
TATCAAC621550.034.4110872
TAACGCA7650.034.3719374
ATCAACG627100.034.0398333
TCAACGC638700.034.0297784
AACGCAG656650.033.7838136
CAACGCA645650.033.6655435
ATAGTAC14100.032.6350564
TAGTACT14800.031.8835075
TCACGCA10600.031.571484
ACGCAGA709600.031.2548457
CGCAGAG717100.031.1028828
GCAGAGT736650.030.2250049
ATCACGC11500.029.1006683
GTACAAC14750.028.7747551
CATGGGG110350.028.4856514
Produced by FastQC (version 0.11.5)