FastQCFastQC Report
Fri 10 Feb 2017
SRR3559421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3884372
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT931982.3993067605265406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439481.1314055399431362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303930.7824430821764754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158800.4088176930530855No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148070.3811941801660603No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA110070.28336626873018345No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96130.24747887174554856No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84080.21645712614548762No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71180.1832471246317294No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.17320689161594202No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45720.11770242396969188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA423400.048.8150671
CGCATAG7800.047.5228461
TATCACG5050.042.602652
TATCAAC525500.039.325822
ATCAACG533800.038.669563
TCAACGC543400.038.558294
CAACGCA550000.038.183995
AACGCAG559950.038.1135066
TACAACG6750.035.414552
ACGCAGA610450.034.9409687
CGCAGAG619300.034.4802258
CATGGGG115900.033.4646874
GCAGAGT640350.033.2365079
TAGTACT12050.031.7240095
CATGGGT51300.031.2207224
TAACGCA7200.029.8810254
GGACCGT8450.028.9815946
AGTACTT390650.028.52679412-13
ATAGTAC13250.028.4151654
GGTATCA253150.027.8210011