FastQCFastQC Report
Fri 10 Feb 2017
SRR3559417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553777
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705112.761047656079603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348701.3654285397667847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228850.8961236631076244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133610.523185853737425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133480.5226768038086332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87660.34325628275295766No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56290.22041861916682623No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.20651764034212852No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43980.17221550667893087No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28720.11246087657614584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27720.1085451078931324No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.10650890817796542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA242050.060.1035351
TATCACG4350.050.643522
CGCATAG8400.048.405271
TATCAAC310350.046.8111722
TAACGCA5400.046.3091664
ATCAACG315100.046.0677223
TCAACGC326050.045.2510384
TACAACG5550.045.0575682
CAACGCA330800.044.6677175
AACGCAG343350.044.264616
ACGCAGA379200.040.0954637
CGCAGAG384200.039.74398
GGTATCA118100.039.0361941
GTATAAC7700.038.8277551
GCAGAGT400250.037.9867559
ATAGTAC11000.036.8067674
ACAACGC6650.035.813753
GTACAAC8200.035.00181
TAGTACT12950.033.518615
AGATTAC11050.031.7907812