FastQCFastQC Report
Fri 10 Feb 2017
SRR3559414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10848401
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3427933.159848165642107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1588621.464381709341312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1049260.9672024476233871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579690.5343552473770098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281010.25903356632926827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266260.24543709252635482No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264760.24405440027521108No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT237910.2193042089797381No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221470.20414990190720272No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC189380.17456950568106766No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA142050.13094095618331217No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT137380.12663617430808466No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC137210.12647946918628836No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT116890.10774859815746117No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG113440.10456840597983057No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA110350.10172005994247446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1007250.075.564391
GGTATCA374650.063.9621
TATCAAC1349800.056.5147362
ATCAACG1392400.054.640293
CAACGCA1429350.053.362425
TCAACGC1429500.053.3513344
AACGCAG1466600.052.154956
ACGCAGA1625250.046.9759987
CGCAGAG1644850.046.438358
GCAGAGT1718050.044.2936489
GACCGTT27100.036.8613437
TACTGGT95200.036.2151032
GTACTGG108900.035.2012941
AGAGTAC1689250.033.85524710-11
GGACCGT41800.033.2866976
GGTTCAC102650.032.6701626
ACTGGTT104550.032.4637683
AGTACTT1232500.031.33425312-13
TTAGGAC37950.029.9688243
GAGTACT1149450.029.41848612-13
Produced by FastQC (version 0.11.5)