FastQCFastQC Report
Fri 10 Feb 2017
SRR3559413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4755024
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1202852.5296402289452167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554311.1657354410829472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374150.7868519696220251No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220360.463425631500493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187150.3935837127215341No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122950.25856862131505537No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99950.21019872875510198No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95060.19991486898909447No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90350.1900095562083388No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC79020.16618212652554434No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT77230.1624176870610958No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66680.14023062764772584No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA59290.12468917086433214No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT57150.1201886678174495No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC57020.1199152727725454No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG48560.10212356446571036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA495150.055.7801061
TAACGCA9300.047.3869864
TATCAAC625850.044.0766532
ATCAACG637900.043.178693
TCAACGC653300.042.76254
CAACGCA656100.042.747125
CGCATAG11200.042.1862831
AACGCAG676300.042.156536
GTATAAC13950.039.443531
ACGCAGA740050.038.5250477
CGCAGAG749800.038.0803958
GCAGAGT774400.036.694129
AGATTAC20300.032.857332
GTACTGG41250.032.622761
ATAACGC17000.032.5794943
GGTATCA265050.032.358141
CATGGGT56350.031.6000374
TACTGGT35050.031.093772
TATCACG7800.030.540472
TACAACG10250.030.2127172