FastQCFastQC Report
Fri 10 Feb 2017
SRR3559409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4801320
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1038292.1625094765606123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469920.9787308490165205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328120.6833953995984438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180550.3760424216673748No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121760.25359692751160096No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89530.1864695542059267No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82010.17080719468812744No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.13917006156640258No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51660.10759541126190299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA358950.064.572851
TATCAAC468650.049.3586772
ATCAACG478300.048.325493
TCAACGC491600.047.417764
CAACGCA496050.047.1799935
AACGCAG511450.046.526696
GGTATCA165900.043.1467321
ACGCAGA568750.041.766077
CGCAGAG579450.041.015358
GCAGAGT602100.039.3341679
CGCATAG9850.032.176571
TAACGCA7700.031.7040124
AGAGTAC592500.030.13164910-11
TACAACG8200.029.7708422
AGTACTT412200.029.13158612-13
GTACATG358450.028.010591
TACATGG362350.027.5894812
ACATGGG347750.027.5835113
GAGTACT382600.027.36797312-13
CATGGGG151300.026.9177234
Produced by FastQC (version 0.11.5)