FastQCFastQC Report
Fri 10 Feb 2017
SRR3559397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6392650
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT803191.2564273032310544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370950.5802757854723785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259320.4056533675392834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120730.1888575160535928No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.10222677606313502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA363150.062.798631
TATCAAC481250.047.0588842
ATCAACG481500.046.935533
TCAACGC491750.046.5020144
CAACGCA494750.046.3431975
AACGCAG514400.045.3487936
GGTATCA170650.043.4253431
ACGCAGA574850.040.5489777
CGCAGAG589050.039.6020748
GCAGAGT625400.037.148169
TATCACG9400.036.7343182
CGCATAG11150.032.159131
ACAACGC12300.031.4615483
GTACATG438050.031.2966331
ACATGGG426800.031.0228863
TACATGG448200.030.5910172
TAACGCA10350.030.4864854
CATGGGG197800.029.6771684
AGAGTAC606200.029.55527510-11
AGTACTT394900.027.57812912-13
Produced by FastQC (version 0.11.5)