FastQCFastQC Report
Fri 10 Feb 2017
SRR3559396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3757844
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726761.9339812935289489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343610.9143806927589331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243260.6473392721996974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116140.3090601951544556No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82010.21823684006041763No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT74640.19862453044884246No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65380.1739827411675418No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58190.15484942961975004No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT58000.15434382055242313No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56820.15120372213428765No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55370.14734512662047705No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT55090.14660001852125848No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA54080.1439123071633628No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45940.12225095027893654No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.10671012420952013No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37790.10056298239096674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA382950.050.6681631
TAACGCA9000.042.3455734
TATCAAC476400.040.7739452
ATCAACG481900.040.172663
CAACGCA500300.039.6585665
AACGCAG518350.039.448116
TCAACGC500750.039.4452824
TATCACG6750.037.934582
CATGGGT53400.037.8032384
GTATAAC11950.037.528041
TCACGCA7500.037.317044
ACGCAGA564050.036.051697
CGCAGAG567450.035.9618958
GCAGAGT587750.034.5478449
TACAACG6500.033.896822
CGCATAG11900.033.6659131
GGACCGT13850.033.4858976
TACTGGT30650.033.2227972
TCTAACG5650.032.672612
GTACTGG34250.032.6470261