FastQCFastQC Report
Fri 10 Feb 2017
SRR3559391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686789
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1853652.1338724815348917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT863700.9942684230041734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556300.6403977349973621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326310.3756393760686486No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226710.2609825103384001No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175830.20241081025451405No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146080.1681634030710312No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138190.15908064533396632No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC93430.1075541261563968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA626300.067.651681
TATCAAC828000.051.1126142
ATCAACG836100.050.5177353
TCAACGC856300.049.8406374
CAACGCA863200.049.574325
AACGCAG888000.048.7533846
GGTATCA271750.044.409181
ACGCAGA984150.043.887547
CGCAGAG1001450.043.2184458
GCAGAGT1050000.041.0955249
TAACGCA11750.034.4622154
AGAGTAC1025000.031.8677910-11
CATGGGG285550.031.6981244
CGCATAG15900.030.8416711
AGTACTT728400.029.68896512-13
ACATGGG620200.029.582313
GTACATG642250.029.5452141
TACATGG648250.029.3127382
GAGTACT671700.027.5433112-13
CAGAGTA1045250.026.73933410-11
Produced by FastQC (version 0.11.5)